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KMID : 0613820190290020158
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Journal of Life Science
2019 Volume.29 No. 2 p.158 ~ p.163
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Isolation of an Agarase-producing Persicobacter sp. DH-3 and Characterization of its β-agarase
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Heo Da-Hye
Lee Dong-Geun
Lee Sang-Hyeon
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Abstract
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The purpose of this study was to isolate a new marine agarase-producing bacterium. Agarase can hydrolyze agar and agarose to produce agarooligosaccharides or neoagarooligosaccharides, which possess many physiological functions. Strain DH-3 was isolated from seawater collected from the coast of Yeosu at Jeollanam province, Korea. A 16S rDNA sequence analysis showed this strain to be Persicobacter sp. DH-3. Extracellular agarase was prepared from culture media of Persicobacter sp. DH-3 and used for characterization. Relative activities at 20, 30, 40, 50, 60, and 70℃ were 50, 55, 70, 100, 90, and 50%, respectively. Relative activities at pH 5, 6, 7, and 8 were 75, 100, 90, and 75%, respectively. The enzyme showed maximum activity at 50℃ in a 20 mM Tris-HCl buffer at pH 6. This enzyme could be useful, as agar is in liquid state at 50℃. Agarase activities were maintained at 80% or more for 2 hr at 20, 30, and 40℃. Thin layer chromatography analysis suggested that Persicobacter sp. DH-3 produced extracellular β-agarases as it hydrolyzed agarose to produce neoagarohexaose and neoagarotetraose. In addition, zymogram analysis confirmed that Persicobacter sp. DH-3 produces at least three agar-degrading enzymes with molecular weights of 45, 70, and 140 kDa. Therefore, it is expected that agarases from Persicobacter sp. DH-3 could be used to produce functional neoagarooligosaccharides.
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KEYWORD
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Agar-degrading bacterium, β-agarase, neoagarooligosaccharides, Persicobacter sp. DH-3, zymogram
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